Construct: shRNA TRCN0000008332

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012193.2-1818s1c1
DNA Barcode:
GCAGAAGTGTTCCAACAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FZD4 (8322)
Gene Description:
frizzled class receptor 4
Transcript:
RefSeq NM_012193.2 (NON-CURRENT)
Match location:
Position 1818 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008332)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGTGTTCCAACAGATT
Hairpin Sequence:
5'-CCGG-GCAGAAGTGTTCCAACAGATT-CTCGAG-AATCTGTTGGAACACTTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGTGTTCCAACAGATTCTCGAGAATCTGTTGGAACACTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGTGTTCCAACAGATTCTCGAGAATCTGTTGGAACACTTCTGC-3'

Other clones with same target sequence:

(none)