Construct: shRNA TRCN0000008423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013842.2-710s1c1
DNA Barcode:
TCTACCCAGAAGGACCTAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xbp1 (22433)
Gene Description:
X-box binding protein 1
Transcript:
RefSeq NM_013842.2 (NON-CURRENT)
Match location:
Position 710 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTACCCAGAAGGACCTAGTT
Hairpin Sequence:
5'-CCGG-TCTACCCAGAAGGACCTAGTT-CTCGAG-AACTAGGTCCTTCTGGGTAGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTACCCAGAAGGACCTAGTTCTCGAGAACTAGGTCCTTCTGGGTAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTACCCAGAAGGACCTAGTTCTCGAGAACTAGGTCCTTCTGGGTAGA-3'

Other clones with same target sequence:

  1. TRCN0000232020