Construct: shRNA TRCN0000008446

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011670.1-44s1c1
DNA Barcode:
GCTGAACAAAGTGTTGGCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uchl1 (22223)
Gene Description:
ubiquitin carboxy-terminal hydrolase L1
Transcript:
RefSeq NM_011670.1 (NON-CURRENT)
Match location:
Position 44 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008446)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAACAAAGTGTTGGCCAA
Hairpin Sequence:
5'-CCGG-GCTGAACAAAGTGTTGGCCAA-CTCGAG-TTGGCCAACACTTTGTTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAACAAAGTGTTGGCCAACTCGAGTTGGCCAACACTTTGTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAACAAAGTGTTGGCCAACTCGAGTTGGCCAACACTTTGTTCAGC-3'

Other clones with same target sequence:

(none)