Construct: shRNA TRCN0000008484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144884.1-468s1c1
DNA Barcode:
GCCTCTAACATCACACAGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tor1a (30931)
Gene Description:
torsin family 1, member A (torsin A)
Transcript:
RefSeq NM_144884.1 (NON-CURRENT)
Match location:
Position 468 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTAACATCACACAGTAT
Hairpin Sequence:
5'-CCGG-GCCTCTAACATCACACAGTAT-CTCGAG-ATACTGTGTGATGTTAGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTAACATCACACAGTATCTCGAGATACTGTGTGATGTTAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTAACATCACACAGTATCTCGAGATACTGTGTGATGTTAGAGGC-3'

Other clones with same target sequence:

(none)