Construct: shRNA TRCN0000008514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_207062.2-369s1c1
DNA Barcode:
GCTGACGAAGATGAAGGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hspa1a (193740)
Gene Description:
heat shock protein 1A
Transcript:
RefSeq XM_207062.2 (NON-CURRENT)
Match location:
Position 369 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACGAAGATGAAGGAGAT
Hairpin Sequence:
5'-CCGG-GCTGACGAAGATGAAGGAGAT-CTCGAG-ATCTCCTTCATCTTCGTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACGAAGATGAAGGAGATCTCGAGATCTCCTTCATCTTCGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACGAAGATGAAGGAGATCTCGAGATCTCCTTCATCTTCGTCAGC-3'

Other clones with same target sequence:

(none)