Construct: shRNA TRCN0000008633

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000356.1-1495s1c1
DNA Barcode:
GAGTCATCAGACAGCAGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCOF1 (6949)
Gene Description:
treacle ribosome biogenesis factor 1
Transcript:
RefSeq NM_000356.1 (NON-CURRENT)
Match location:
Position 1495 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008633)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTCATCAGACAGCAGTGAT
Hairpin Sequence:
5'-CCGG-GAGTCATCAGACAGCAGTGAT-CTCGAG-ATCACTGCTGTCTGATGACTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTCATCAGACAGCAGTGATCTCGAGATCACTGCTGTCTGATGACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTCATCAGACAGCAGTGATCTCGAGATCACTGCTGTCTGATGACTC-3'

Other clones with same target sequence:

  1. TRCN0000342854