Construct: shRNA TRCN0000008683

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006058.2-521s1c1
DNA Barcode:
GCTCACAGGAAAGGACTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNIP1 (10318)
Gene Description:
TNFAIP3 interacting protein 1
Transcript:
RefSeq NM_006058.2 (NON-CURRENT)
Match location:
Position 521 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008683)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACAGGAAAGGACTCAAA
Hairpin Sequence:
5'-CCGG-GCTCACAGGAAAGGACTCAAA-CTCGAG-TTTGAGTCCTTTCCTGTGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACAGGAAAGGACTCAAACTCGAGTTTGAGTCCTTTCCTGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACAGGAAAGGACTCAAACTCGAGTTTGAGTCCTTTCCTGTGAGC-3'

Other clones with same target sequence:

(none)