Construct: shRNA TRCN0000008701

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_078483.2-1150s1c1
DNA Barcode:
CCTGCAATTTGGAGCTAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC36A1 (206358)
Gene Description:
solute carrier family 36 member 1
Transcript:
RefSeq NM_078483.2 (NON-CURRENT)
Match location:
Position 1150 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008701)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCAATTTGGAGCTAATAT
Hairpin Sequence:
5'-CCGG-CCTGCAATTTGGAGCTAATAT-CTCGAG-ATATTAGCTCCAAATTGCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCAATTTGGAGCTAATATCTCGAGATATTAGCTCCAAATTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCAATTTGGAGCTAATATCTCGAGATATTAGCTCCAAATTGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000229647