Construct: shRNA TRCN0000008710

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003118.1-879s1c1
DNA Barcode:
CCTGGACAATGACAAGTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPARC (6678)
Gene Description:
secreted protein acidic and cysteine rich
Transcript:
RefSeq NM_003118.1 (NON-CURRENT)
Match location:
Position 879 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008710)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGACAATGACAAGTACAT
Hairpin Sequence:
5'-CCGG-CCTGGACAATGACAAGTACAT-CTCGAG-ATGTACTTGTCATTGTCCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGACAATGACAAGTACATCTCGAGATGTACTTGTCATTGTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGACAATGACAAGTACATCTCGAGATGTACTTGTCATTGTCCAGG-3'

Other clones with same target sequence:

(none)