Construct: shRNA TRCN0000008730

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024529.3-504s1c1
DNA Barcode:
GCTCCCTTAGAAATAGGTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC73 (79577)
Gene Description:
cell division cycle 73
Transcript:
RefSeq NM_024529.3 (NON-CURRENT)
Match location:
Position 504 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008730)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCCTTAGAAATAGGTCTT
Hairpin Sequence:
5'-CCGG-GCTCCCTTAGAAATAGGTCTT-CTCGAG-AAGACCTATTTCTAAGGGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCCTTAGAAATAGGTCTTCTCGAGAAGACCTATTTCTAAGGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCCTTAGAAATAGGTCTTCTCGAGAAGACCTATTTCTAAGGGAGC-3'

Other clones with same target sequence:

(none)