Construct: shRNA TRCN0000008735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138792.2-182s1c1
DNA Barcode:
CCAAGTAATAAGGAACTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LEO1 (123169)
Gene Description:
LEO1 homolog, Paf1/RNA polymerase II complex component
Transcript:
RefSeq NM_138792.2 (NON-CURRENT)
Match location:
Position 182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008735)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGTAATAAGGAACTGTTT
Hairpin Sequence:
5'-CCGG-CCAAGTAATAAGGAACTGTTT-CTCGAG-AAACAGTTCCTTATTACTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGTAATAAGGAACTGTTTCTCGAGAAACAGTTCCTTATTACTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGTAATAAGGAACTGTTTCTCGAGAAACAGTTCCTTATTACTTGG-3'

Other clones with same target sequence:

  1. TRCN0000329685