Construct: shRNA TRCN0000008778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005494.2-339s1c1
DNA Barcode:
CGGGACATCTATGACAAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAJB6 (10049)
Gene Description:
DnaJ heat shock protein family (Hsp40) member B6
Transcript:
RefSeq NM_005494.2 (NON-CURRENT)
Match location:
Position 339 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGACATCTATGACAAATAT
Hairpin Sequence:
5'-CCGG-CGGGACATCTATGACAAATAT-CTCGAG-ATATTTGTCATAGATGTCCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGACATCTATGACAAATATCTCGAGATATTTGTCATAGATGTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGACATCTATGACAAATATCTCGAGATATTTGTCATAGATGTCCCG-3'

Other clones with same target sequence:

(none)