Construct: shRNA TRCN0000008795

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004246.1-1044s1c1
DNA Barcode:
GCTCTGTGTAACAGTCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLP2R (9340)
Gene Description:
glucagon like peptide 2 receptor
Transcript:
RefSeq NM_004246.1 (NON-CURRENT)
Match location:
Position 1044 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008795)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGTGTAACAGTCAATTT
Hairpin Sequence:
5'-CCGG-GCTCTGTGTAACAGTCAATTT-CTCGAG-AAATTGACTGTTACACAGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGTGTAACAGTCAATTTCTCGAGAAATTGACTGTTACACAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGTGTAACAGTCAATTTCTCGAGAAATTGACTGTTACACAGAGC-3'

Other clones with same target sequence:

(none)