Construct: shRNA TRCN0000008800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005272.2-1290s1c1
DNA Barcode:
CCTCAGGTATAAGTTCTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNAT2 (2780)
Gene Description:
G protein subunit alpha transducin 2
Transcript:
RefSeq NM_005272.2 (NON-CURRENT)
Match location:
Position 1290 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGGTATAAGTTCTATAA
Hairpin Sequence:
5'-CCGG-CCTCAGGTATAAGTTCTATAA-CTCGAG-TTATAGAACTTATACCTGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGGTATAAGTTCTATAACTCGAGTTATAGAACTTATACCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGGTATAAGTTCTATAACTCGAGTTATAGAACTTATACCTGAGG-3'

Other clones with same target sequence:

(none)