Construct: shRNA TRCN0000008836

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016602.1-89s1c1
DNA Barcode:
GAGCTTTGCTACAAGGCCGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCR10 (2826)
Gene Description:
C-C motif chemokine receptor 10
Transcript:
RefSeq NM_016602.1 (NON-CURRENT)
Match location:
Position 89 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008836)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTTTGCTACAAGGCCGAT
Hairpin Sequence:
5'-CCGG-GAGCTTTGCTACAAGGCCGAT-CTCGAG-ATCGGCCTTGTAGCAAAGCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTTTGCTACAAGGCCGATCTCGAGATCGGCCTTGTAGCAAAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTTTGCTACAAGGCCGATCTCGAGATCGGCCTTGTAGCAAAGCTC-3'

Other clones with same target sequence:

(none)