Construct: shRNA TRCN0000008888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005301.2-1391s1c1
DNA Barcode:
CGCCATCTGCTACTACTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR35 (2859)
Gene Description:
G protein-coupled receptor 35
Transcript:
RefSeq NM_005301.2 (NON-CURRENT)
Match location:
Position 1391 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCATCTGCTACTACTACAT
Hairpin Sequence:
5'-CCGG-CGCCATCTGCTACTACTACAT-CTCGAG-ATGTAGTAGTAGCAGATGGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCATCTGCTACTACTACATCTCGAGATGTAGTAGTAGCAGATGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCATCTGCTACTACTACATCTCGAGATGTAGTAGTAGCAGATGGCG-3'

Other clones with same target sequence:

(none)