Construct: shRNA TRCN0000008914

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144773.2-69s1c1
DNA Barcode:
CCTCTCCTTTAACTTCAGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PROKR2 (128674)
Gene Description:
prokineticin receptor 2
Transcript:
RefSeq NM_144773.2 (NON-CURRENT)
Match location:
Position 69 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008914)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCCTTTAACTTCAGTTA
Hairpin Sequence:
5'-CCGG-CCTCTCCTTTAACTTCAGTTA-CTCGAG-TAACTGAAGTTAAAGGAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCCTTTAACTTCAGTTACTCGAGTAACTGAAGTTAAAGGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCCTTTAACTTCAGTTACTCGAGTAACTGAAGTTAAAGGAGAGG-3'

Other clones with same target sequence:

(none)