Construct: shRNA TRCN0000008967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023915.2-622s1c1
DNA Barcode:
CCTCATAATGACGCTGACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR87 (53836)
Gene Description:
G protein-coupled receptor 87
Transcript:
RefSeq NM_023915.2 (NON-CURRENT)
Match location:
Position 622 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATAATGACGCTGACATT
Hairpin Sequence:
5'-CCGG-CCTCATAATGACGCTGACATT-CTCGAG-AATGTCAGCGTCATTATGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATAATGACGCTGACATTCTCGAGAATGTCAGCGTCATTATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATAATGACGCTGACATTCTCGAGAATGTCAGCGTCATTATGAGG-3'

Other clones with same target sequence:

(none)