Construct: shRNA TRCN0000008982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016235.1-1566s1c1
DNA Barcode:
CGCAAACTAAAGCAAAGCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPRC5B (51704)
Gene Description:
G protein-coupled receptor class C group 5 member B
Transcript:
RefSeq NM_016235.1 (NON-CURRENT)
Match location:
Position 1566 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAAACTAAAGCAAAGCTAA
Hairpin Sequence:
5'-CCGG-CGCAAACTAAAGCAAAGCTAA-CTCGAG-TTAGCTTTGCTTTAGTTTGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAAACTAAAGCAAAGCTAACTCGAGTTAGCTTTGCTTTAGTTTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAAACTAAAGCAAAGCTAACTCGAGTTAGCTTTGCTTTAGTTTGCG-3'

Other clones with same target sequence:

(none)