Construct: shRNA TRCN0000009098

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000868.1-3359s1c1
DNA Barcode:
GCAGTTATTTACCAGAAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HTR2C (3358)
Gene Description:
5-hydroxytryptamine receptor 2C
Transcript:
RefSeq NM_000868.1 (NON-CURRENT)
Match location:
Position 3359 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009098)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTATTTACCAGAAGTTT
Hairpin Sequence:
5'-CCGG-GCAGTTATTTACCAGAAGTTT-CTCGAG-AAACTTCTGGTAAATAACTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTATTTACCAGAAGTTTCTCGAGAAACTTCTGGTAAATAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTATTTACCAGAAGTTTCTCGAGAAACTTCTGGTAAATAACTGC-3'

Other clones with same target sequence:

(none)