Construct: shRNA TRCN0000009118

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024012.1-117s1c1
DNA Barcode:
CGGAGTGCTTATTCTCACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HTR5A (3361)
Gene Description:
5-hydroxytryptamine receptor 5A
Transcript:
RefSeq NM_024012.1 (NON-CURRENT)
Match location:
Position 117 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009118)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGTGCTTATTCTCACCTT
Hairpin Sequence:
5'-CCGG-CGGAGTGCTTATTCTCACCTT-CTCGAG-AAGGTGAGAATAAGCACTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGTGCTTATTCTCACCTTCTCGAGAAGGTGAGAATAAGCACTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGTGCTTATTCTCACCTTCTCGAGAAGGTGAGAATAAGCACTCCG-3'

Other clones with same target sequence:

(none)