Construct: shRNA TRCN0000009183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005958.2-1564s1c1
DNA Barcode:
GCCTCTTATTACAGAGGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTNR1A (4543)
Gene Description:
melatonin receptor 1A
Transcript:
RefSeq NM_005958.2 (NON-CURRENT)
Match location:
Position 1564 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTTATTACAGAGGGAAA
Hairpin Sequence:
5'-CCGG-GCCTCTTATTACAGAGGGAAA-CTCGAG-TTTCCCTCTGTAATAAGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTTATTACAGAGGGAAACTCGAGTTTCCCTCTGTAATAAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTTATTACAGAGGGAAACTCGAGTTTCCCTCTGTAATAAGAGGC-3'

Other clones with same target sequence:

(none)