Construct: shRNA TRCN0000009337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012360.1-576s1c1
DNA Barcode:
ACACCTCAATGAGGTCATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR1F1 (4992)
Gene Description:
olfactory receptor family 1 subfamily F member 1 (gene/pseudogene)
Transcript:
RefSeq NM_012360.1 (NON-CURRENT)
Match location:
Position 576 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACCTCAATGAGGTCATAAT
Hairpin Sequence:
5'-CCGG-ACACCTCAATGAGGTCATAAT-CTCGAG-ATTATGACCTCATTGAGGTGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACCTCAATGAGGTCATAATCTCGAGATTATGACCTCATTGAGGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACCTCAATGAGGTCATAATCTCGAGATTATGACCTCATTGAGGTGT-3'

Other clones with same target sequence:

(none)