Construct: shRNA TRCN0000009390

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002550.1-791s1c1
DNA Barcode:
GACTGGGTTCAACCAAGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR3A1 (4994)
Gene Description:
olfactory receptor family 3 subfamily A member 1 (gene/pseudogene)
Transcript:
RefSeq NM_002550.1 (NON-CURRENT)
Match location:
Position 791 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009390)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTGGGTTCAACCAAGCTTT
Hairpin Sequence:
5'-CCGG-GACTGGGTTCAACCAAGCTTT-CTCGAG-AAAGCTTGGTTGAACCCAGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTGGGTTCAACCAAGCTTTCTCGAGAAAGCTTGGTTGAACCCAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTGGGTTCAACCAAGCTTTCTCGAGAAAGCTTGGTTGAACCCAGTC-3'

Other clones with same target sequence:

(none)