Construct: shRNA TRCN0000009400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012373.1-49s1c1
DNA Barcode:
GCTGTTGCTGAGTTCATTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR3A3 (8392)
Gene Description:
olfactory receptor family 3 subfamily A member 3
Transcript:
RefSeq NM_012373.1 (NON-CURRENT)
Match location:
Position 49 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTGCTGAGTTCATTCTA
Hairpin Sequence:
5'-CCGG-GCTGTTGCTGAGTTCATTCTA-CTCGAG-TAGAATGAACTCAGCAACAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTGCTGAGTTCATTCTACTCGAGTAGAATGAACTCAGCAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTGCTGAGTTCATTCTACTCGAGTAGAATGAACTCAGCAACAGC-3'

Other clones with same target sequence:

(none)