Construct: shRNA TRCN0000009454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022146.1-222s1c1
DNA Barcode:
GCATACTGTCACCAACATGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPFFR1 (64106)
Gene Description:
neuropeptide FF receptor 1
Transcript:
RefSeq NM_022146.1 (NON-CURRENT)
Match location:
Position 222 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATACTGTCACCAACATGTT
Hairpin Sequence:
5'-CCGG-GCATACTGTCACCAACATGTT-CTCGAG-AACATGTTGGTGACAGTATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATACTGTCACCAACATGTTCTCGAGAACATGTTGGTGACAGTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATACTGTCACCAACATGTTCTCGAGAACATGTTGGTGACAGTATGC-3'

Other clones with same target sequence:

(none)