Construct: shRNA TRCN0000009479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176876.1-762s1c1
DNA Barcode:
CGGTCTAGTCTGGCATGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
P2RY12 (64805)
Gene Description:
purinergic receptor P2Y12
Transcript:
RefSeq NM_176876.1 (NON-CURRENT)
Match location:
Position 762 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTCTAGTCTGGCATGAAAT
Hairpin Sequence:
5'-CCGG-CGGTCTAGTCTGGCATGAAAT-CTCGAG-ATTTCATGCCAGACTAGACCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTCTAGTCTGGCATGAAATCTCGAGATTTCATGCCAGACTAGACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTCTAGTCTGGCATGAAATCTCGAGATTTCATGCCAGACTAGACCG-3'

Other clones with same target sequence:

(none)