Construct: shRNA TRCN0000009513

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008689.1-1583s1c1
DNA Barcode:
GCACCATAAACACCAAATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nfkb1 (18033)
Gene Description:
nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105
Transcript:
RefSeq NM_008689.1 (NON-CURRENT)
Match location:
Position 1583 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009513)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCATAAACACCAAATTTA
Hairpin Sequence:
5'-CCGG-GCACCATAAACACCAAATTTA-CTCGAG-TAAATTTGGTGTTTATGGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCATAAACACCAAATTTACTCGAGTAAATTTGGTGTTTATGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCATAAACACCAAATTTACTCGAGTAAATTTGGTGTTTATGGTGC-3'

Other clones with same target sequence:

(none)