Construct: shRNA TRCN0000009592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172400.1-1032s1c1
DNA Barcode:
CCACGCCTCTAACTTAATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnajc8 (68598)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C8
Transcript:
RefSeq NM_172400.1 (NON-CURRENT)
Match location:
Position 1032 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGCCTCTAACTTAATGTT
Hairpin Sequence:
5'-CCGG-CCACGCCTCTAACTTAATGTT-CTCGAG-AACATTAAGTTAGAGGCGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGCCTCTAACTTAATGTTCTCGAGAACATTAAGTTAGAGGCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGCCTCTAACTTAATGTTCTCGAGAACATTAAGTTAGAGGCGTGG-3'

Other clones with same target sequence:

  1. TRCN0000345089