Construct: shRNA TRCN0000009609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010228.2-2321s1c1
DNA Barcode:
GCCTCAGATCACTTGGTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Flt1 (14254)
Gene Description:
FMS-like tyrosine kinase 1
Transcript:
RefSeq NM_010228.2 (NON-CURRENT)
Match location:
Position 2321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCAGATCACTTGGTTCAA
Hairpin Sequence:
5'-CCGG-GCCTCAGATCACTTGGTTCAA-CTCGAG-TTGAACCAAGTGATCTGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCAGATCACTTGGTTCAACTCGAGTTGAACCAAGTGATCTGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCAGATCACTTGGTTCAACTCGAGTTGAACCAAGTGATCTGAGGC-3'

Other clones with same target sequence:

(none)