Construct: shRNA TRCN0000009657

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177367.2-616s1c1
DNA Barcode:
CCATCCTCCAAAGAGGTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gemin4 (276919)
Gene Description:
gem nuclear organelle associated protein 4
Transcript:
RefSeq NM_177367.2 (NON-CURRENT)
Match location:
Position 616 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009657)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCCTCCAAAGAGGTTTAA
Hairpin Sequence:
5'-CCGG-CCATCCTCCAAAGAGGTTTAA-CTCGAG-TTAAACCTCTTTGGAGGATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCTCCAAAGAGGTTTAACTCGAGTTAAACCTCTTTGGAGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCTCCAAAGAGGTTTAACTCGAGTTAAACCTCTTTGGAGGATGG-3'

Other clones with same target sequence:

(none)