Construct: shRNA TRCN0000009757

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008105.2-375s1c1
DNA Barcode:
CCTGTGGAGAAACAAACTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gcnt2 (14538)
Gene Description:
glucosaminyl (N-acetyl) transferase 2, I-branching enzyme
Transcript:
RefSeq NM_008105.2 (NON-CURRENT)
Match location:
Position 375 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009757)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGGAGAAACAAACTAAT
Hairpin Sequence:
5'-CCGG-CCTGTGGAGAAACAAACTAAT-CTCGAG-ATTAGTTTGTTTCTCCACAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGGAGAAACAAACTAATCTCGAGATTAGTTTGTTTCTCCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGGAGAAACAAACTAATCTCGAGATTAGTTTGTTTCTCCACAGG-3'

Other clones with same target sequence:

(none)