Construct: shRNA TRCN0000009777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_138596.2-989s1c1
DNA Barcode:
GCTTAATAACTCGCTGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sycp2l (218175)
Gene Description:
synaptonemal complex protein 2-like
Transcript:
RefSeq XM_138596.2 (NON-CURRENT)
Match location:
Position 989 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAATAACTCGCTGCTTTA
Hairpin Sequence:
5'-CCGG-GCTTAATAACTCGCTGCTTTA-CTCGAG-TAAAGCAGCGAGTTATTAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAATAACTCGCTGCTTTACTCGAGTAAAGCAGCGAGTTATTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAATAACTCGCTGCTTTACTCGAGTAAAGCAGCGAGTTATTAAGC-3'

Other clones with same target sequence:

(none)