Construct: shRNA TRCN0000009824

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007294.x-7237s1c1
DNA Barcode:
TATAGCTGTTGGAAGGACTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRCA1 (672)
Gene Description:
BRCA1 DNA repair associated
Transcript:
RefSeq NM_007294.1 (NON-CURRENT)
Match location:
Position 7237 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009824)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATAGCTGTTGGAAGGACTAG
Hairpin Sequence:
5'-CCGG-TATAGCTGTTGGAAGGACTAG-CTCGAG-CTAGTCCTTCCAACAGCTATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATAGCTGTTGGAAGGACTAGCTCGAGCTAGTCCTTCCAACAGCTATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATAGCTGTTGGAAGGACTAGCTCGAGCTAGTCCTTCCAACAGCTATA-3'

Other clones with same target sequence:

(none)