Construct: shRNA TRCN0000009838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005732.x-1051s1c1
DNA Barcode:
AGATTCGTGATCAGATTACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAD50 (10111)
Gene Description:
RAD50 double strand break repair protein
Transcript:
RefSeq NM_005732.2 (NON-CURRENT)
Match location:
Position 1051 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATTCGTGATCAGATTACA
Hairpin Sequence:
5'-ACCG-GAGATTCGTGATCAGATTACA-CTCGAG-TGTAATCTGATCACGAATCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATTCGTGATCAGATTACACTCGAGTGTAATCTGATCACGAATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATTCGTGATCAGATTACACTCGAGTGTAATCTGATCACGAATCTC-3'

Other clones with same target sequence:

(none)