Construct: shRNA TRCN0000009843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005544.x-3313s1c1
DNA Barcode:
AGTTCAGAGATCGTTCCTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRS1 (3667)
Gene Description:
insulin receptor substrate 1
Transcript:
RefSeq NM_005544.1 (NON-CURRENT)
Match location:
Position 3313 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTCAGAGATCGTTCCTAT
Hairpin Sequence:
5'-ACCG-GAGTTCAGAGATCGTTCCTAT-CTCGAG-ATAGGAACGATCTCTGAACTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTCAGAGATCGTTCCTATCTCGAGATAGGAACGATCTCTGAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTCAGAGATCGTTCCTATCTCGAGATAGGAACGATCTCTGAACTC-3'

Other clones with same target sequence:

(none)