Construct: shRNA TRCN0000009857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004064.x-1752s1c1
DNA Barcode:
AATGGTGATCACTCCAGGTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKN1B (1027)
Gene Description:
cyclin dependent kinase inhibitor 1B
Transcript:
RefSeq NM_004064.2 (NON-CURRENT)
Match location:
Position 1752 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGGTGATCACTCCAGGTA
Hairpin Sequence:
5'-ACCG-GAATGGTGATCACTCCAGGTA-CTCGAG-TACCTGGAGTGATCACCATTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGGTGATCACTCCAGGTACTCGAGTACCTGGAGTGATCACCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGGTGATCACTCCAGGTACTCGAGTACCTGGAGTGATCACCATTC-3'

Other clones with same target sequence:

(none)