Construct: shRNA TRCN0000009883

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001429.x-7731s1c1
DNA Barcode:
CAATTCCGAGACATCTTGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EP300 (2033)
Gene Description:
E1A binding protein p300
Transcript:
RefSeq NM_001429.1 (NON-CURRENT)
Match location:
Position 7731 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009883)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATTCCGAGACATCTTGAGA
Hairpin Sequence:
5'-CCGG-CAATTCCGAGACATCTTGAGA-CTCGAG-TCTCAAGATGTCTCGGAATTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATTCCGAGACATCTTGAGACTCGAGTCTCAAGATGTCTCGGAATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATTCCGAGACATCTTGAGACTCGAGTCTCAAGATGTCTCGGAATTG-3'

Other clones with same target sequence:

(none)