Construct: shRNA TRCN0000009888
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_004119.x-3309s1c1
- DNA Barcode:
- AATTGTGTACCTGAAGTACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FLT3 (2322)
- Gene Description:
- fms related tyrosine kinase 3
- Transcript:
- RefSeq NM_004119.1 (NON-CURRENT)
- Match location:
- Position 3309 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000009888)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAATTGTGTACCTGAAGTACA
- Hairpin Sequence:
-
5'-ACCG-GAATTGTGTACCTGAAGTACA-CTCGAG-TGTACTTCAGGTACACAATTC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAATTGTGTACCTGAAGTACACTCGAGTGTACTTCAGGTACACAATTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAATTGTGTACCTGAAGTACACTCGAGTGTACTTCAGGTACACAATTC-3'
Other clones with same target sequence:
(none)
