Construct: shRNA TRCN0000009892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008066.x-775s1c1
DNA Barcode:
AGTCCAAGCCGAATGTCCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabra2 (14395)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2
Transcript:
RefSeq NM_008066.2 (NON-CURRENT)
Match location:
Position 775 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCCAAGCCGAATGTCCCAT
Hairpin Sequence:
5'-CCGG-AGTCCAAGCCGAATGTCCCAT-CTCGAG-ATGGGACATTCGGCTTGGACT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCCAAGCCGAATGTCCCATCTCGAGATGGGACATTCGGCTTGGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCCAAGCCGAATGTCCCATCTCGAGATGGGACATTCGGCTTGGACT-3'

Other clones with same target sequence:

(none)