Construct: shRNA TRCN0000009894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008067.x-460s1c1
DNA Barcode:
CTTCGACCTGGACTTGGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabra3 (14396)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit alpha 3
Transcript:
RefSeq NM_008067.2 (NON-CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCGACCTGGACTTGGAGAT
Hairpin Sequence:
5'-CCGG-CTTCGACCTGGACTTGGAGAT-CTCGAG-ATCTCCAAGTCCAGGTCGAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCGACCTGGACTTGGAGATCTCGAGATCTCCAAGTCCAGGTCGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCGACCTGGACTTGGAGATCTCGAGATCTCCAAGTCCAGGTCGAAG-3'

Other clones with same target sequence:

(none)