Construct: shRNA TRCN0000009899

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176942.x-506s1c1
DNA Barcode:
GACGGACTCTTGGATGGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabra5 (110886)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit alpha 5
Transcript:
RefSeq NM_176942.2 (NON-CURRENT)
Match location:
Position 506 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009899)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGGACTCTTGGATGGCTAT
Hairpin Sequence:
5'-CCGG-GACGGACTCTTGGATGGCTAT-CTCGAG-ATAGCCATCCAAGAGTCCGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGGACTCTTGGATGGCTATCTCGAGATAGCCATCCAAGAGTCCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGGACTCTTGGATGGCTATCTCGAGATAGCCATCCAAGAGTCCGTC-3'

Other clones with same target sequence:

(none)