Construct: shRNA TRCN0000009924

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_148938.x-815s1c1
DNA Barcode:
CCATGTGCTTCGGTTTCGTGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc1a3 (20512)
Gene Description:
solute carrier family 1 (glial high affinity glutamate transporter), member 3
Transcript:
RefSeq NM_148938.1 (NON-CURRENT)
Match location:
Position 815 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009924)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTGCTTCGGTTTCGTGA
Hairpin Sequence:
5'-CCGG-CCATGTGCTTCGGTTTCGTGA-CTCGAG-TCACGAAACCGAAGCACATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTGCTTCGGTTTCGTGACTCGAGTCACGAAACCGAAGCACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTGCTTCGGTTTCGTGACTCGAGTCACGAAACCGAAGCACATGG-3'

Other clones with same target sequence:

(none)