Construct: shRNA TRCN0000009991

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002758.x-901s1c1
DNA Barcode:
CAATGCTCTCGGTCAAGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K6 (5608)
Gene Description:
mitogen-activated protein kinase kinase 6
Transcript:
RefSeq NM_002758.2 (NON-CURRENT)
Match location:
Position 901 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000009991)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATGCTCTCGGTCAAGTGAA
Hairpin Sequence:
5'-CCGG-CAATGCTCTCGGTCAAGTGAA-CTCGAG-TTCACTTGACCGAGAGCATTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATGCTCTCGGTCAAGTGAACTCGAGTTCACTTGACCGAGAGCATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATGCTCTCGGTCAAGTGAACTCGAGTTCACTTGACCGAGAGCATTG-3'

Other clones with same target sequence:

  1. TRCN0000350353