Construct: shRNA TRCN0000010022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005546.x-430s1c1
DNA Barcode:
GGTGCCTAAATATCATCCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITK (3702)
Gene Description:
IL2 inducible T cell kinase
Transcript:
RefSeq NM_005546.3 (NON-CURRENT)
Match location:
Position 430 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTGCCTAAATATCATCCTAA
Hairpin Sequence:
5'-CCGG-GGTGCCTAAATATCATCCTAA-CTCGAG-TTAGGATGATATTTAGGCACC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTGCCTAAATATCATCCTAACTCGAGTTAGGATGATATTTAGGCACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTGCCTAAATATCATCCTAACTCGAGTTAGGATGATATTTAGGCACC-3'

Other clones with same target sequence:

(none)