Construct: shRNA TRCN0000010152

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004302.x-872s1c1
DNA Barcode:
TGTGGCTTGTTTCTGACTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACVR1B (91)
Gene Description:
activin A receptor type 1B
Transcript:
RefSeq NM_004302.2 (NON-CURRENT)
Match location:
Position 872 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010152)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGGCTTGTTTCTGACTATC
Hairpin Sequence:
5'-CCGG-TGTGGCTTGTTTCTGACTATC-CTCGAG-GATAGTCAGAAACAAGCCACA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGGCTTGTTTCTGACTATCCTCGAGGATAGTCAGAAACAAGCCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGGCTTGTTTCTGACTATCCTCGAGGATAGTCAGAAACAAGCCACA-3'

Other clones with same target sequence:

(none)