Construct: shRNA TRCN0000010177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005356.x-230s1c1
DNA Barcode:
CACTGCAAGACAACCTGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LCK (3932)
Gene Description:
LCK proto-oncogene, Src family tyrosine kinase
Transcript:
RefSeq NM_005356.2 (NON-CURRENT)
Match location:
Position 230 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTGCAAGACAACCTGGTTA
Hairpin Sequence:
5'-CCGG-CACTGCAAGACAACCTGGTTA-CTCGAG-TAACCAGGTTGTCTTGCAGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTGCAAGACAACCTGGTTACTCGAGTAACCAGGTTGTCTTGCAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTGCAAGACAACCTGGTTACTCGAGTAACCAGGTTGTCTTGCAGTG-3'

Other clones with same target sequence:

  1. TRCN0000055434