Construct: shRNA TRCN0000010180

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005443.x-1490s1c1
DNA Barcode:
GGCCATCTTCCCATCTCCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAPSS1 (9061)
Gene Description:
3'-phosphoadenosine 5'-phosphosulfate synthase 1
Transcript:
RefSeq NM_005443.2 (NON-CURRENT)
Match location:
Position 1490 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010180)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCCATCTTCCCATCTCCCAT
Hairpin Sequence:
5'-CCGG-GGCCATCTTCCCATCTCCCAT-CTCGAG-ATGGGAGATGGGAAGATGGCC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCCATCTTCCCATCTCCCATCTCGAGATGGGAGATGGGAAGATGGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCCATCTTCCCATCTCCCATCTCGAGATGGGAGATGGGAAGATGGCC-3'

Other clones with same target sequence:

  1. TRCN0000320508