Construct: shRNA TRCN0000010186

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005465.x-569s1c1
DNA Barcode:
TGGCACACACTCTAACTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKT3 (10000)
Gene Description:
AKT serine/threonine kinase 3
Transcript:
RefSeq NM_005465.2 (NON-CURRENT)
Match location:
Position 569 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010186)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCACACACTCTAACTGAAA
Hairpin Sequence:
5'-CCGG-TGGCACACACTCTAACTGAAA-CTCGAG-TTTCAGTTAGAGTGTGTGCCA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCACACACTCTAACTGAAACTCGAGTTTCAGTTAGAGTGTGTGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCACACACTCTAACTGAAACTCGAGTTTCAGTTAGAGTGTGTGCCA-3'

Other clones with same target sequence:

(none)