Construct: shRNA TRCN0000010200

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005884.x-285s1c1
DNA Barcode:
CGACCAGCACGAGCAGAAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK4 (10298)
Gene Description:
p21 (RAC1) activated kinase 4
Transcript:
RefSeq NM_005884.2 (NON-CURRENT)
Match location:
Position 285 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000010200)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCAGCACGAGCAGAAGTT
Hairpin Sequence:
5'-CCGG-CGACCAGCACGAGCAGAAGTT-CTCGAG-AACTTCTGCTCGTGCTGGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCAGCACGAGCAGAAGTTCTCGAGAACTTCTGCTCGTGCTGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCAGCACGAGCAGAAGTTCTCGAGAACTTCTGCTCGTGCTGGTCG-3'

Other clones with same target sequence:

  1. TRCN0000272579